Cell Culture and Tissue Engineering Laboratory

Objectives

The subject Cell Cultures and Tissue Engineering Laboratory, with a high practical component, introduces students to 3D cultures and shows them some basic steps of tissue engineering, such as 3D printing of matrices artificial or decellularization as a model for generating natural matrices. In addition, the students are also introduced to tissue regeneration / tissue engineering through the completion of a project. This subject is the natural continuation of the second-year subject of the degree in Biomedicine, Cell Cultures and Tissue Engineering , in which students have already familiarized themselves mainly with the theoretical concepts of this discipline and have also a practical part of introduction to cell cultures.

Learning outcomes

• RA1. Learn about cell and tissue culture techniques.
• LO2. Performs monolayer cultures of continuous cell lines correctly and knows the main methods of tissue engineering.
• LO3. It applies imaging techniques to the analysis of the functioning of the organism at different hierarchical levels.
• LO4. Globally evaluates the learning processes carried out in accordance with the plans and objectives set and establishes measures for individual improvement.
• LO5. It fully includes oral and written messages of different types expressed in Catalan, Spanish and English.
• LO6. Acquire and demonstrate advanced knowledge of the theoretical and practical aspects and working methodology of the field of biomedicine.
• LO7. Moves with ease in complex situations or situations that require the development of new solutions.
• LO8. It collects and interprets data and information on which it can base its conclusions, which include reflections on matters of a social, scientific or ethical nature in the field of biomedicine.

Skills

General skills

• Formulate hypotheses following the scientific method, with an ability to summarize and analyze information in a critical way in order to be able to solve problems.

Specific skills

• Promote, respect and apply the principles of bioethics in professional activity.
• Recognize the organization, structure and function of cells and their components in different tissues.
• Use key analytical and imaging techniques, and basic technological instruments, following customary preclinical research laboratory protocols.

Basic skills

• Students can apply their knowledge to their work or vocation in a professional manner and have competencies typically demonstrated through drafting and defending arguments and solving problems in their field of study.
• Students have demonstrated knowledge and understanding in a field of study that builds on general secondary education with the support of advanced textbooks and knowledge of the latest advances in this field of study.
• Students have the ability to gather and interpret relevant data (usually within their field of study) in order to make judgments that include reflection on relevant social, scientific and ethical issues.

Core skills

• Communicate orally, in writing and audiovisually, in one's own language and in foreign languages, with proficiency in form, content and use.
• Reflect critically on knowledge of all kinds, with a commitment to professional rigor and quality.

Content

In this subject the students make 2D crops again to generate and monitor 3D crops.

In the field of tissue engineering, 3D printing is introduced to generate artificial matrices or scaffolds , and also in the decellularization of animal tissue to generate natural matrices or scaffolds .

In addition, the students participate in a team work or project to find the solution for the regeneration of different tissues.

Evaluation

• Observation of participation, attitude and attendance: 10%
• Specific assessment tests related to practices: 40%. To be able to make an average with the rest of the activities, the minimum grade must reach 4.5.
• Completion of the work or project: 35%
• Follow-up of the work done (students will have to complete the different phases of the project indicated by the teaching staff): 15%

Attendance at internships is mandatory:

• Absences are not allowed, but you can be absent for justified reasons. More than 2 unexcused absences involve suspending the entire subject.
• Justified absences do not exclude students from doing the work derived from the session they did not attend.

The following are considered excused absences :

• Hospital admission
• Court summons
• Death of a relative of the 1st or 2nd degree of consanguinity or affinity
• Medically diagnosed illness that makes it impossible to attend the session
• Attendance at a scheduled hospital visit that cannot be postponed and involves diagnosis, follow-up and/or medical treatment

In the event of a justified absence :

• If there is an option, the student must retake the class in another group.
• If the class cannot be made up, the student must do an assignment (suggested by the teaching staff) that allows them to make up, in whole or in part, the content of the session they missed. The preparation of this work is mandatory and failure to submit the work involves a penalty equivalent to the reduction of 5% of the final mark of the subject.

In the case of unexcused absence :

• The class cannot be retaken and a penalty equivalent to the reduction of 10% of the subject's final grade is applied for each session missed. (Therefore, one absence implies a reduction of 10% of the final mark, and two, one of 20%.)
• Absence from 3 sessions or more implies suspending the subject.
• The student can do recommended work to recover, in whole or in part, the content of the missed session, but it is not mandatory. If he does the work satisfactorily he can recover 5% of the penalty imposed for the absence.

important

Plagiarism or copying someone else's work is penalized at all universities and, according to the UVic-UCC Coexistence Rules , constitutes serious or very serious offences. For this reason, in the course of this subject, plagiarism or the misappropriation of other people's texts or ideas (see what is considered plagiarism ) and the improper or undeclared use of artificial intelligence in an activity are translated automatically in suspension or other disciplinary measures.

To cite texts and materials appropriately, consult the academic citation guidelines and guidelines available on the UVic Library website.

Methodology

The methodology used in this subject combines:

• Teamwork in the laboratory
• Realization of a team project

Bibliography

Key references

• Panagiotis, M. (2019). Stem Cell and Biologic Scaffold Engineering. Retrieved from https://directory.doabooks.org/handle/20.500.12854/60016
• Radwa, A.M. (2019). Cell Culture. Retrieved from https://directory.doabooks.org/handle/20.500.12854/130722
• Xianquan, Z. (2022). Cell Culture: Advanced Technology and Applications in Medical and Life Sciences. Retrieved from https://directory.doabooks.org/handle/20.500.12854/90341

Further reading

Teachers will provide complementary bibliography and compulsory reading throughout the course via the Virtual Campus.