Cell Culture and Tissue Engineering Laboratory

Objectives

The Cell Culture and Tissue Engineering Laboratory subject, with a high practical component, introduces students to 3D cultures and shows them some basic steps of tissue engineering, such as 3D printing of artificial matrices or decellularization as a model for generating natural matrices. In addition, students are also introduced to tissue regeneration / tissue engineering through the completion of a project. This subject is the natural continuation of the second year subject of the degree in Biomedicine, Cell Cultures and Tissue Engineering, in which students have already become familiar with the theoretical concepts of this discipline and have also completed a practical introduction to cell cultures.

Learning outcomes

• LO1. Knows cell and tissue culture techniques.
• LO2. Correctly carries out monolayer cultures of continuous cell lines and knows the main methods of tissue engineering.
• LO3. It applies imaging techniques to the analysis of the functioning of the organism at different hierarchical levels.
• LO4. Evaluates globally the learning processes carried out in accordance with the plans and objectives set and establishes individual improvement measures.
• LO5. Understands completely oral and written messages of different types expressed in Catalan, Spanish and English.
• LO6. Acquires and demonstrates advanced knowledge of the theoretical and practical aspects and working methodology of the field of biomedicine.
• RA7. Moves with ease in complex situations or those that require the development of new solutions.
• LO8. Collects and interprets data and information on which to base their conclusions, which include reflections on social, scientific or ethical issues in the field of biomedicine.

Competencies

General skills

• Formulate hypotheses following the scientific method, with an ability to summarize and analyze information in a critical way in order to be able to solve problems.

Specific skills

• Promote, respect and apply the principles of bioethics in professional activity.
• Recognize the organization, structure and function of cells and their components in different tissues.
• Use key analytical and imaging techniques, and basic technological instruments, following customary preclinical research laboratory protocols.

Basic skills

• Students can apply their knowledge to their work or vocation in a professional manner and have competencies typically demonstrated through drafting and defending arguments and solving problems in their field of study.
• Students have demonstrated knowledge and understanding in a field of study that builds on general secondary education with the support of advanced textbooks and knowledge of the latest advances in this field of study.
• Students have the ability to gather and interpret relevant data (usually within their field of study) in order to make judgments that include reflection on relevant social, scientific and ethical issues.

Core skills

• Communicate orally, in writing and audiovisually, in one's own language and in foreign languages, with proficiency in form, content and use.
• Reflect critically on knowledge of all kinds, with a commitment to professional rigor and quality.

Content

In this subject, students once again create 2D crops to generate and track 3D crops.

In the field of tissue engineering, 3D printing is introduced to generate matrices or scaffolding artificial, and also in the decellularization of animal tissue for the generation of matrices or scaffolding natural.

In addition, students participate in a team project or work to find the solution for the regeneration of different tissues.

Evaluation

• Observation of participation, attitude and attendance: 10%
• Specific assessment tests related to the practices: 40%. To be able to average with the rest of the activities, the minimum grade must reach 4.5.
• Completion of the work or project: 35%
• Monitoring of the work carried out (students must complete the different phases of achieving the project indicated by the teacher): 15%

Attendance at internships is mandatory:

• Absences are not permitted, but you can be absent for justified reasons. More than 2 unexcused absences will result in failing the entire subject.
• Excused absences do not exclude students from doing the work derived from the session they did not attend.

They are considered excused absences:

• Hospital admission
• Judicial summons
• Death of a relative of the 1st or 2nd degree of consanguinity or affinity
• Medically diagnosed illness that prevents attendance at the session
• Attendance at a scheduled hospital visit that cannot be postponed and that involves diagnosis, monitoring and/or medical treatment

In case of a excused absence:

• If there is an option, the student must retake the class in another group.
• If the class cannot be made up, the student must do an assignment (proposed by the professor) that allows them to recover, in whole or in part, the content of the session they missed. The preparation of this assignment is mandatory and failure to submit the assignment will result in a penalty equivalent to a 5% reduction in the final grade for the subject.

In the case ofunexcused absence:

• The class cannot be made up and a penalty equivalent to a 10% reduction in the final grade of the subject will be applied for each session missed. (Therefore, one absence means a 10% reduction in the final grade, and two, a 20% reduction.)
• Absence from 3 or more sessions implies failing the subject.
• The student may do a recommended assignment to recover, in whole or in part, the content of the session they missed, but it is not mandatory. If they do the assignment satisfactorily, they can recover 5% of the penalty imposed for the absence.

Important

Plagiarism or copying someone else's work is penalized in all universities and, according to the UVic-UCC coexistence rules, constitute serious or very serious faults. Therefore, during the course of this subject, plagiarism or the improper appropriation of texts or ideas from other people (see What is considered plagiarism?) and the improper or undeclared use of artificial intelligence in an activity automatically result in a suspension or other disciplinary measures.

To cite texts and materials appropriately, you must consult the academic citation guidelines and guidelines available on the UVic Library website.

Methodology

The methodology used in this subject combines:

• Teamwork in the laboratory
• Carrying out a team project

Bibliography

Key references

• Panagiotis, M. (2019). Stem Cell and Biologic Scaffold Engineering. Retrieved from https://directory.doabooks.org/handle/20.500.12854/60016
• Radwa, A.M. (2019). Cell Culture. Retrieved from https://directory.doabooks.org/handle/20.500.12854/130722
• Xianquan, Z. (2022). Cell Culture: Advanced Technology and Applications in Medical and Life Sciences. Retrieved from https://directory.doabooks.org/handle/20.500.12854/90341

Further reading

Teachers will provide complementary bibliography and compulsory reading throughout the course via the Virtual Campus.