Course
Biology
Subject
Molecular Biology Techniques
Type
Optional (OP)
Credits
3.0
Semester
1st
Group | Language of instruction | Teachers |
---|---|---|
G11, classroom instruction, mornings | English | Josep Bau Macià |
David Pujal Bau |
Sustainable Development Goals (SDG)
- 14. Life below water
- 15. Life on land
Objectives
The Molecular Biology Techniques subject is taught in a project-based learning (PBL) format.
The experimental project consists in the identification of an organism through the DNA barcoding technique, which is based on the amplification and sequencing of a mitochondrial gene fragment: Cytochrom oxidase I (CO1).
Throughout the project, several molecular biology techniques are introduced, discussed and applied, while reinforcing different technical and cross-curricular skills.
Subject aims
- Observe at all times the safety and operation regulations in the laboratory.
- Manipulate properly the routine-use laboratory instruments and correctly apply microbiology and molecular biology protocols.
- Record in a proper and orderly manner all the activities carried out in the laboratory.
- Understand and successfully apply protocols for extraction, amplification and analysis of nucleic acids.
- Carry out the molecular identification of a species of insect from a tissue sample.
Sustainable Developement Goals
This subject addresses the study of global and local biodiversity in the context of the following SDGs:
- Goal 14. Life Below Water - Conserve and sustainably use the oceans, seas and marine resources
- Goal 15. Life On Land - Biodiversity, Forests, Desertification
Learning outcomes
- LO1. Gains autonomy and initiative in the laboratory.
- LO2. Properly uses routine-use instruments in a biological laboratory, including safety and disposal regulations.
- LO3. Plans the execution and carries out an experimental protocol in a team-work context and in a suitable time.
- LO4. Keeps an adequate activity record and issues reports that justify and analyze the work done.
- LO5. Performs a critical interpretation of the experimental results to draw reasonable conclusions.
- LO6. Understands the basics of basic molecular biology techniques and applies them correctly.
- LO7. Searches the necessary bibliographical resources.
- LO8. Understands and is able to communicate complex oral and written messages.
- LO9. Writes written reports and documents with proper spelling and grammar.
Competencies
Specific skills
- Have oral and written skills in English for communicating results, conclusions and processes deriving from research in the field of biology.
- Manipulate databases used in the field of biology.
- Master basic laboratory techniques in biology, apply protocols and use appropriate instruments, observing safety norms and correctly interpreting the results obtained.
- Understand the processes of functional integration in organisms based on knowledge of subcellular structure and organism cell types.
Basic skills
- Students can apply their knowledge to their work or vocation in a professional manner and have competencies typically demonstrated through drafting and defending arguments and solving problems in their field of study.
- Students have demonstrated knowledge and understanding in a field of study that builds on general secondary education with the support of advanced textbooks and knowledge of the latest advances in this field of study.
Core skills
- Be a critical thinker before knowledge in all its dimensions. Show intellectual, cultural and scientific curiosity and a commitment to professional rigour and quality.
- Use oral, written and audiovisual forms of communication, in one's own language and in foreign languages, with a high standard of use, form and content.
Content
- Introduction to laboratory work
- Basic laboratory regulations
- Reagent manipulation and waste disposal
- Team work organisation
- Introduction to the Barcode of Life Project: Molecular-based identification of species
- Sample collection
- Gathering and organisation of sample metadata
- Amplification and sequencing of cytochrome oxidase I (CO1)
- PCR amplification
- Sanger sequencing
- Data analysis
- Data pre-processing and quality check
- Sequence alignment and identification
- Introduction to the phylogenetic analysis of sequences
Evaluation
Assessment activities (which represent 100% of the final mark (F.M.))
- Activity 1: Written test (40% of the F.M.). Minimum grade: 4/10. Resit allowed
- Activity 2: Team work preparation of a public exposition (15% of the F.M.). Resit not allowed
- Activity 3: Exercises and final report (30% of F.M.). Resit not allowed (late submission penalises 20%)
- Activity 4: Personal performance and attitude in the laboratory (15% of the F.M.). Resit not allowed
Additional considerations
- Attendance at all sessions is mandatory. Justified absence up to a maximum of 20% of sessions.
- Unjustified absence to more than 20% of the sessions (or justified absence to more than 40%) results in a grade of 0/10 of activity 4.
- Absence to more than 40% of the practical activities implies failing the subject.
- The lack of punctuality will be assessed negatively and, if it is repeated and unjustified, it will be considered absence.
- Activity 2: Unjustified absence to final exposition results in a grade of 0/10 and a penalisation of 25% on the grade obtained by the group to which the student belongs.
- Activity 4 will assess the following aspects:
- Proper work in the laboratory and correct use and care of basic materials and techniques.
- Understanding and correct application of the laboratory protocols.
- Results obtained in the practical experiments.
Methodology
- The methodology of the project is based on the development of an eminently hands-on laboratory activity.
- Various resources and activities, both guided and self-study, are contemplated, which must allow students the adequate achievement of the competences, skills and knowledge associated with the protocols that will be developed throughout the sessions.
Bibliography
Key references
- W. John Kress and David L. Erickson, Editors (2012). DNA Barcodes: Methods and Protocols. Springer.
Further reading
Teachers will provide complementary bibliography and compulsory reading throughout the course via the Virtual Campus.